A Bit of History...
High-resolution melting analysis is a new method introduced in 2003. Similar to the LightCycler, this too was a result of collaboration between our lab at the University of Utah and Idaho Technology, Inc (Salt Lake City, UT). The method uses high data-density acquisition, and detects small sequence differences in PCR fragments, just by direct melting. Melting curves can now be used for mutation scanning, sequence matching, and mutliplex genotyping - analyses that traditionally required processing of PCR products by electrophoresis or other non-homogeneous means. Because of its speed and simplicity, popularity of this method is growing.
Two inventions were key to high-resolution melting: 1) high-resolution instruments, and 2) novel saturation dyes. To read more, please follow the colored tabs in the navigation bar (top of page) ..
Click Link or Preview Image to Launch Tutorial in New Window !
Key references Contact us to receive reprints.
|© 2006- Wittwer Lab. All Rights Reserved.|