NonHuman Polyploid Organisms

Gene
Order
Species
Method
Ploidy
# Samp
Amplicons
Dye
Inst
# Variants
Accuracy
Title
Author
Year
Link
Email
Length
#
Min
Max
%
#
Plant
P. dulcis
Scanning
2
25
4
68
198
SYTO 9
RG
17
100
25
High resolution melting analysis of almond SNPs derived from ESTs
Wu
2008
 
 
Plant
M. sativa
Scanning
4
191
2
40
200
LCGreen Plus
LS
2
87
13
Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and..
Han
2011
 
 
Parasite
Clonorchis..
Amp
2
14
1
165
165
HRM
LC480
1
100
14
Molecular Differentiation of Opisthorchis viverrini and Clonorchis..
Kaewkong
2013
 
 
Parasite
Schistosoma..
Detection
2
0
2
156
156
ResoLight
LC480
2
0
0
Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi..
Kongklieng
2013
 
 
Parasite
Wuchereria..
Amp
2
14
1
161
227
ResoLight
LC480
4
100
14
Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi,..
Thanchomnang
2013
 
 
Parasite
Babesia canis
Amp Typing
2
800
2
0
0
SYBR Green I
RG
7
100
158
The factors affecting the distribution of babesiosis in dogs in Poland
Adaszek
2011
 
 
Parasite
Plasmodium..
Amp
2
88
1
165
165
EvaGreen
CFX96
2
98
86
Rapid Discrimination between Anopheles gambiae s.s. and Anopheles..
Zianni
2013
 
 
Parasite
Opisthorchis..
Amp
2
7
1
209
209
ResoLight
LC480
1
100
7
Molecular Differentiation of Opisthorchis viverrini and Clonorchis..
Kaewkong
2013
 
 
Plant
Prototheca..
Amp
10
1
250
250
SYBR Green
RG
1
100
10
Rapid Molecular Typing of Prototheca zopfii by High Resolution Melting..
Sobukawa
2013
 
 
Parasite
Filarial..
Detection
1, 2
2039
1
115
115
ResoLight
LC480
3
0
0
A high resolution melting real time PCR for mapping of filaria infection..
Wongkamchai
2013
 
 
Animal
Mollusca,..
UL
2
6
3042
100
100
LCGreen Plus
LS
1
0
0
Large-Scale Development of Gene-Associated Single-Nucleotide..
Jiao
2013
 
 
Parasite
D. immitis, D...
Amp
2
15
1
256
256
EvaGreen
Eco
2
100
15
Rapid differentiation of Dirofilaria immitis and Dirofilariarepens in..
Albonico
2013
 
 
Plant
Plant Oil
Detection
2
9
1
71
71
SYTO 9
7900HT
9
0
0
Detection of plant oil DNA using high resolution melting (HRM) post PCR..
Vietina
2013
 
 
Fungi
(Sporothrix..
Amp
1, 2
455
1
0
0
SYBR Green
RG
3
0
0
High-resolution DNA melt-curve analysis for cost-effective mass screening..
McCarthy
2013
 
 
Fish
Kokanee salmon
Amp
2
88
36
68
171
EvaGreen
CFX96
0
0
0
Transcriptome-wide comparison of sequence variation in divergent ecotypes..
Lemay
2013
 
 
Plant
Alfalfa
Amp
2
192
20
51
170
LCGreen Plus
LS
0
0
0
Prevalence of single nucleotide polymorphism among 27 diverse alfalfa..
Li
2012
 
 
Plant
Citrus
Amp
2
15
24
0
0
SYTO 9
RG
0
100
360
High Resolution Melting Analysis Is a More Sensitive and Effective..
Distefano
2012
 
 
Plant
Prunus avium..
Detection
2
8
223
0
0
SYBR Green I
LC480
8
0
0
Rapid gene-based SNP and haplotype marker development in non-model..
Koepke
2012
 
 
Fungi
Acanthamoeba..
Amp
38
2
204
210
SYTO 9
ABI 7500
10
70
53
New Strategy for Rapid Diagnosis and Characterization of Keratomycosis
Goldschmidt
2012
 
 
Fish
Pacific..
Amp
2
14
14
0
0
ResoLight
LC480
0
0
0
Development of Genomic Resources for Pacific Herring through Targeted..
Roberts
2012
 
 
Parasite
Leishmania..
2
208
2
265
273
0
0
0
Leishmania tropica Infection in Golden Jackals and Red Foxes, Israel
Talmi-Frank
2010
 
 
Plant
Arabidopsis..
Amp
2
0
3
0
0
RG
0
0
0
A simple, high throughput method to locate single copy sequences from..
Vu
2010
 
 
Plant
Potato,..
Amp
2
0
7
100
300
LCGreen Plus
LS
0
0
0
A High-Resolution Melting Approach for Analyzing Allelic Expression..
Yuan
2009
 
 
Insect
Drosophila..
2
0
2
0
0
LCGreen Plus
LS
0
0
0
CRISPR/Cas9 mediated genome engineering in Drosophila
Bassett
2014
 
 
Fungi
Eight Candida..
Amp
2
19
2
0
0
SYTO 9
Eco
0
0
0
The development and validation of a rapid genetic method for species..
Alnuaimi
2014
 
 
Plant
Pummelo..
Amp
2
260
25
35
61
LCGreen Plus
LS
0
0
0
Identification of Pummelo Cultivars by Using a Panel of 25 Selected SNPs..
Wu
2014
 
 
Animal
Sheep
Amp
2
24
4
0
0
LCGreen Plus
LS
0
0
0
Limitation of high-resolution melting curve analysis for genotyping simple..
Yang
2014
 
 
Bacteria
Enterococcus..
2
62
0
0
0
RG
0
97
60
Evaluation of High-Resolution Melting Curve Analysis of Ligation- Mediated..
Woksepp
2014
 
 
Oyster
C. gigas
Amp
2
32
377
60
104
SYTO 9
LC480
0
0
0
SNP Mining in Crassostrea gigas EST Data: Transferability to Four Other..
Zhong
2014
 
 
Plant
P ginseng and..
Amp
2
4
34
18
0
EvaGreen
CFX96
0
0
0
De novo transcriptome assembly and the identification of..
Jo
2015
 
 
Plant
Alfalfa
Amp
2
13
0
0
0
S82
LC480
0
0
0
Identification of Molecular Markers Associated with Verticillium Wilt..
Zhang
2014
 
 
Plant
Apple
0
0
0
0
ResoLight
LC480
0
0
0
Application of the high-resolution melting technique for gene mapping and..
Chagné
2015
 
 
12S and 18S RNA
Animal
buffalo,..
Detection
2
30
2
77
220
SYTO 9
RG
5
0
0
A fast and accurate method for controlling the correct labeling of..
Sakaridis
2013
 
 
12S rRNA
Parasite
B. malayi, B...
Amp
2
48
1
115
115
ResoLight
LC480
3
100
48
Rapid Detection and Identification of Brugia malayi, B. pahangi, and..
Wongkamchai
2013
 
 
12S rRNA and..
Animal
11 species
Amp
2
118
2
0
0
EvaGreen
RG
0
0
0
High-Resolution Melting of 12S rRNA and Cytochrome b DNA Sequences for..
Naue
2014
 
 
12S-rRNA
Animal
10 species
Amp
2
137
10
93
399
EvaGreen
RG
0
100
137
Reliable Discrimination of 10 Ungulate Species Using High Resolution..
Ramon-Laca
2014
 
 
139 genes
Animal
Bulls
Detection
2
42
407
0
0
SYTO 9
ABI 7500
1613
0
0
High-resolution melt analysis does not reveal mutagenic risk in sexed..
Pozzi
2014
 
 
159 genes
Plant
Pea
Amp
2
2
0
0
0
ResoLight
RG
2
0
0
Combining gene expression and genetic analyses to identify candidate genes..
Legrand
2013
 
 
16S rDNA
Bacteria
Bacillus..
Amp
1, 2
237
2
70
100
EvaGreen
RG
7
100
474
Rapid identification of dairy mesophilic and thermophilic sporeforming..
Chauhan
2013
 
 
16S rRNA
Bacteria
Anaplasma..
Amp
2
42
1
257
257
EvaGreen
Biorad
2
100
42
A Novel High-Resolution Melt PCR Assay Discriminates Anaplasma..
Krucken
2013
 
 
16S rRNA
Bacteria
Various
Detection
70
3
0
0
LCGreen Plus
HR-96
0
90
63
Comparative Analysis of Two Broad-Range PCR Assays for Pathogen Detection..
Jeng
2012
 
 
16S rRNA
Bacteria
Lactic Acid..
Amp
Various
86
1
0
0
ResoLight
LC480
13
0
0
Strain-level characterization of nonstarter lactic acid bacteria in..
Porcellato
2012
 
 
16S rRNA
Bacteria
Various
Detection
106
3
0
0
LCGreen Plus
LS
21
70.6
75
Identification of Bacterial Pathogens in Ascitic Fluids from Patients with..
Hardick
2012
 
 
16S rRNA
Bacteria
Various
Detection
21
2
150
200
ResoLight
LC480
0
0
0
Combined Molecular Gram Typing and High- Resolution Melting Analysis for..
Ozbak
2012
 
 
16S rRNA
Bacteria
Various
Amp
13
2
0
0
ResoLight
LC480
13
77
10
Rapid lactic acid bacteria identification in dairy products by..
Porcellato
2012
 
 
16S rRNA
Bacteria, Fungi
Various
0
0
0
0
SYTO 9,..
HR-1, RG, LC, LS
0
0
0
The potential of high resolution melting analysis (hrma) to streamline,..
Ruskova
2011
 
 
16S rRNA,..
Algae
cyanobacterial..
Detection
2
206
0
0
0
RG
5
100
0
Rapid, multiplex-tandem PCR assay for automated detection and..
Baker
2013
 
 
16SG rRNA
Animal
Koala
Amp
2
288
1
0
0
RG
0
0
0
The prevalence and clinical significance of Chlamydia infection in island..
Patterson
2015
 
 
18S
Parasite
Babesia canis..
Amp Typing
2
20
1
559
559
SYBR Green I
2
0
0
Application of the SYBR Green real-time HRM PCR technique in the..
Adaszek
2010
 
 
18S
Bacteria, Fungi
Staphylococcus..
Amp
1, 2
8
2
180
210
SYTO 9
ABI 7500
0
0
0
Enzymatic Treatment of Specimens before DNA Extraction Directly Influences..
Goldschmidt
2014
 
 
18S rDNA
Fungus
Mucormycosis
Amp
31
0
0
0
EvaGreen
RG
0
0
0
Rapid Detection and Identification of Mucormycetes from Culture and Tissue..
Hrncirova
2010
 
 
18S rRNA
Fungi
Various
Amp
10
2
162
270
SYTO 9
ABI 7500
12
0
0
New Strategy for Rapid Diagnosis and Characterization of Fungal..
Goldschmidt
2012
 
 
18S rRNA
Parasite
Babesia..
Detection
2
299
0
0
0
0
0
0
Frequency and therapy monitoring of canine Babesia spp. infection by..
Wang
2010
 
 
18S rRNA
Parasite
Plasmodium
Nested
2
197
0
0
0
EvaGreen
RG
0
0
0
High-resolution melting analysis reveals low Plasmodium parasitaemia..
Kipanga
2014
 
 
26S/28S lsrDNA
Parasite
Trichinella
Amp
2
4
1
90
90
EvaGreen
RG
4
100
4
Ribo HRM — Detection of inter- and intra-species polymorphisms within..
Masny
2012
 
 
6msas and idh
Fungi
Penicillium..
Amp
2
21
1
96
96
EvaGreen
CFX96
21
100
21
Genetic structure and natural variation associated with host of origin in..
Sanzani
2013
 
 
7SL RNA
Parasite
Leishmania
Amp Typing
2
269
1
119
119
SYTO 9 (1.5 uM)
RG
4
100
269
Rapid diagnosis of old world Leishmaniasis by high resolution melting..
Nasereddin
2010
 
 
7SL RNA
Parasite
Leishmania..
Amp
2
13
2
0
0
EvaGreen
CFX96
0
0
0
Natural Infection of North African Gundi (Ctenodactylus gundi) by..
Bousslimi
2012
 
 
897 genes
Mollusc
Oyster
Amp
2
60
1671
39
140
LCGreen Plus
HR-96
0
0
0
Discovery and validation of genic single nucleotide polymorphisms in the..
Wang
2015
 
 
ace-2
Insect
Culex pipiens
Amp
2
60
1
76
76
EvaGreen
RG
3
97
58
Identification of Culex complex species using SNP markers based on..
Kang
2013
 
 
all-53308
Scallop
Argopecten..
Amp
2
109
334
0
0
LCGreen Plus
LS-96
0
0
0
SNP Identification by Transcriptome Sequencing and Candidate Gene-Based..
Du
2014
 
 
apc
Fish
Zebrafish
Amp Typing
2
48
1
53
53
LCGreen Plus
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
Apj
Animal
Sea Cucumber..
UL
2
159
200
70
110
LCGreen Plus
HR-96
48
0
0
Development of 101 Gene-based Single Nucleotide Polymorphism Markers in..
Du
2012
 
 
ARF7, Exp,..
Plant
S. lycopersicum
Amp Typing
2
22742
5
220
469
LCGreen Plus
LS
3
99.3
1640
Implementation of two high through-put techniques in a novel application:..
Gady
2009
 
 
ARP, ANT,..
Fish
swordfish..
UL
2
774
10
300
950
LCGreen Plus,..
LC480
2
100
7740
Methodological streamlining of SNP discovery and genotyping via..
Smith
2013
 
 
At5G49430,..
Plant
Medicago..
Amp
2
6
20
100
150
LCGreen Plus
ABI 7500
2
100
120
High-resolution melting analysis for SNP genotyping and mapping in..
Han
2012
 
 
B1
Parasite
Toxoplasma..
Amp Typing
2
14
3
82
481
LCGreen Plus
LC480
3
100
42
Genotyping of the protozoan pathogen Toxoplasma gondii using..
Costa
2011
 
 
B1
Parasite
Toxoplasma..
Amp Typing
2
14
3
82
481
LCGreen Plus
LC480
3
100
42
High-resolution melting for analysis of short sequence repeats in..
Ricchi
2011
 
 
B1, ITS-2
Parasite
Toxoplasma..
Detection
1, 2
53
2
0
0
EvaGreen
CFX96
0
100
106
Detection of Toxoplasma gondii and Cyclospora cayetanensis in Mytilus..
Aksoy
2014
 
 
bags13d21
Plant
Barley
Amp Typing
2
8
1
142
142
SYBR Green I
RG
0
100
8
Temperature Switch PCR (TSP): Robust assay design for reliable..
Tabone
2009
 
 
BAL
Fungi
Rhizopus spp.,..
Detection
2
99
0
0
0
RG
5
0
0
Rapid Detection and Identification of Mucormycetes in Bronchoalveolar..
Lengerova
2014
 
 
bap28
Fish
Zebrafish
Amp Typing
2
48
1
41
46
LCGreen Plus
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
Bbaa2
Animal
Mice
UL
2
0
2
0
0
LCGreen Plus
LC480
0
0
0
High-throughput genotyping of advanced congenic lines by High Resolution..
Bramwell
2012
 
 
BenA and bos1
Fungi
Botrytis..
Amp
2
1
3
73
87
EvaGreen
RG
8
100
3
Development of a two-step high-resolution melting (HRM) analysis for..
Chatzidimopoulo..
2014
 
 
BraA.CAX1a and..
Plant
Brassica rapa.
Amp
2
0
4
0
0
EvaGreen
LS
37
100
0
High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS..
Lochlainn
2011
 
 
C1H21orf62,..
Animal
Datong Yak
Amp
2
101
9
60
100
LCGreen Plus
LS
2
0
0
Associations of single nucleotide polymorphisms in candidate genes with..
Liu
2013
 
 
C3'H, C4H,..
Plant
C. cardunculus
Amp Typing
192
3
63
627
EvaGreen
RG
4
0
0
Genetic map of artichoke x wild cardoon: toward a consensus map for Cynara..
Sonnante
2011
 
 
C9orf72, Fus
Rodent
Mice
Detection
2
10
2
133
140
LCGreen Plus
LS
2
100
20
Highly Efficient Targeted Mutagenesis in Mice Using TALENs
Panda
2013
 
 
CDC3
Fungi
Ca albicans
Amp Typing
2
95
1
94
110
ResoLight
LC480
0
0
0
Genotyping of Candida albicans using length fragment and high-resolution..
Costa
2010
 
 
CDC3
Fungi
yeast
Amp Typing
2
95
1
0
0
ResoLight
LC480
3
0
0
Genotyping of Candida albicans using length fragment and high-resolution..
Costa
2010
 
 
CDC3
Fungi
Candida..
Amp
2
82
1
0
0
LC480
0
0
0
Temporal Similarity Between Candida albicans Genotypes in a Tunisian..
Abdeljelil
2012
 
 
CG14073
Insect
Drosophila
Detection
2
0
0
0
0
LCGreen Plus
LS
2
0
0
Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9..
Bassett
2013
 
 
CHD1
Animal
Bird
Amp
2
111
1
2
44
EvaGreen
CFX96
14
0
0
High-resolution melting analysis for bird sexing: a successful approach to..
Morinha
2013
 
 
Chloroplast..
Plant
Thale Cress
Scanning
2
1
320
350
1330
LCGreen Plus
LC480
0
0
0
A rapid high-throughput method for the detection and quantification of RNA..
Chateigner-Bout..
2007
 
 
Cntg10192
Plant
Lettuce
Amp Typing
2
132
1
185
185
LCGreen Plus
LS
0
100
132
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
Cntg4252
Plant
Lettuce
UL Typing
2
192
1
208
208
LCGreen Plus
LS
0
0
0
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
CO1
Parasite
Schistosoma..
Detection
2
166
1
0
0
ResoLight
LC480
2
100
166
Natural Interactions between S. haematobium and S. guineensis in the..
Mone
2012
 
 
CO1
Insect
Bactericera..
Amp
2
12
2
67
94
SYBR Green I
LC480
0
100
24
Identification of a Fourth Haplotype of Bactericera cockerelli (Hemiptera:..
Swisher
2014
 
 
CO1
Insect
Bactericera..
Amp
2
450
1
500
500
SYBR Green I
LC480
0
100
450
High Resolution Melting Analysis of the Cytochrome Oxidase I Gene..
Swisher
2012
 
 
COI
Insect
L. sericata,..
Amp Typing
15
15
70
119
EvaGreen
RG
15
100
15
Identification of forensically important blowfly species (Diptera:..
Malewski
2010
 
 
COI
Insect
Drosophila..
Amp
2
65
1
150
150
EvaGreen
CFX96
0
0
0
A HRM Real-Time PCR Assay for Rapid and Specific Identification of the..
Dhami
2014
 
 
Col-0, Ler-0
Plant
2
4
0
0
0
LC480
0
0
0
Screening for Imprinted Genes Using High-Resolution Melting Analysis of..
Day
2014
 
 
cox1
Parasite
Echinococcus
Detection
2
107
1
444
444
S13
RG
7
100
107
Rapid detection of Echinococcus species by a high-resolution melting (HRM)..
Santos
2013
 
 
cox1
Parasite
Fascioloides..
Detection
2
200
1
439
439
LCGreen Plus
LS
2
0
0
The origin of the giant liver fluke, Fascioloides magna (Trematoda:..
Bazsalovicsová
2013
 
 
Cre
Rodent
Mice
Detection
2
25
2
82
700
LCGreen Plus
5
0
0
High Resolution Melt Analysis (HRMA); a Viable Alternative to Agarose Gel..
Thomsen
2012
 
 
crt
Parasite
Plasmodium..
UP
2
53
1
145
145
LCGreen Plus
LS
4
100
53
Rapid identification of choloroquine and atovaquone drug resistance in..
Gan
2010
 
 
crt
Parasite
Plasmodium..
Amp Typing
2
59
1
198
198
EvaGreen
RG
2
100
59
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
cry9Da4,..
Bacteria
Bacillus..
Amp
2
48
1
100
100
LCGreen Plus
LS
48
0
0
Characterization of cry9Da4, cry9Eb2, and cry9Ee1 genes from Bacillus..
Shu
2013
 
 
CYP51A
Fungi
As fumigatus
Amp Typing
2
25
1
107
107
EvaGreen
RG
0
100
12
Use of a high-resolution melt assay to characterize codon 54 of the cyp51A..
Tuohy
2010
 
 
cyp51A
Fungi
Aspergillus
UL
2
78
2
354
354
ResoLight
LC480
6
100
156
Rapid Detection and Identification of Aspergillus from Lower Respiratory..
Alonso
2012
 
 
Cyt b
Parasite
Trypanosoma..
Detection
2
36
1
383
383
SYBR Green I
RG
14
100
36
High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful..
Pena
2012
 
 
cyt bc1
Parasite
Plasmodium..
UP
2
53
1
116
116
LCGreen Plus
LS
4
100
53
Rapid identification of choloroquine and atovaquone drug resistance in..
Gan
2010
 
 
CytB
Parasite
Trypanosoma..
Detection
2
71
1
0
0
SYBR Green I
RG
0
0
0
Eco-Epidemiology of Chagas Disease in an Endemic Area of Colombia: Risk..
Pena-Garcia
2014
 
 
dhfr
Parasite
Plasmodium..
Amp Typing
2
203
3
167
200
EvaGreen
RG
2
98
203
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
dhps
Parasite
Plasmodium..
Amp Typing
2
87
2
181
195
EvaGreen
RG
2
89
87
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
DLOOP
Cheese
Feta
Amp
15
2
77
145
SYTO 9
RG
0
0
0
A novel closed-tube method based on high resolution melting (HRM) analysis..
Ganopoulos
2013
 
 
DMD
Rodent
? Mdx
Amp Typing
?
46
1
84
84
SYTO 9
RG
2
96
44
A novel and simple method for genotyping the mdx mouse using..
Trebbin
2009
 
 
DREB
Plant
Triticum..
Detection
2
1
10
0
100
EvaGreen
RG
5
100
10
Allelic variants in durum wheat (Triticum turgidum L. var. durum) DREB..
Mondini
2014
 
 
DREB1, HKT1,..
Plant
Durum Wheat..
Amp
2
48
4
100
100
EvaGreen
RG
4
100
192
Identification of SNP Mutations in DREB1, HKT1, and WRKY1 Genes Involved..
Mondini
2012
 
 
eif2b5
Fish
Zebrafish
2
0
0
0
0
0
0
0
Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution..
Xing
2014
 
 
eIF4E
Plant
Barley
Detection
2
643
7
130
620
LCGreen Plus
LS
30
100
4501
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid..
Hofinger
2009
 
 
elF4E
Plant
H. vulgare
Amp Typing
2
643
7
130
620
LCGreen Plus
LS
10
100
44
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid..
Hofinger
2009
 
 
ESTs..
Plant
Cr japonica
Amp Typing
2
8
574
90
120
EvaGreen
LC480
0
0
0
An efficient method for developing SNP markers based on EST data combined..
Ujino-Ihara
2010
 
 
FcCB
Shrimp
Fenneropenaeus..
UL
2
192
3
0
0
LCGreen Plus
HR-96
2
0
0
Molecular cloning and characterization of a cathepsin B gene from the..
Li
2013
 
 
FcERK
Shrimp
Fenneropenaeus..
UL
2
192
2
0
0
LCGreen Plus
HR96
1
0
0
Identification, cloning and characterization of an extracellular..
Li
2013
 
 
FOXP2
Fish
Zebrafish
Amp
2
25
1
0
0
LCGreen Plus
LS-96
1
0
0
Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding
Xing
2012
 
 
Gln1-3
Plant
Maize
Detection
2
8902
4
0
0
LCGreen Plus,..
LS
3
0
0
A Cost-effective High-resolution Melting Approach using the EvaGreen Dye..
Li
2010
 
 
Glyma16g34070,..
Plant
Soybean
UL
2
334
1
129
129
ResoLight
LC480
0
0
0
Genetic mapping of the powdery mildew resistance gene in soybean PI 567301B
Jun
2012
 
 
golden, ryr3,..
Fish
Zebrafish
Amp
2
1
6
90
120
LCGreen Plus
LS
1
0
0
Simple Methods for Generating and Detecting Locus- Specific Mutations..
Dahlem
2012
 
 
Gp-9
Insect
S. invicta
Amp Typing
100
3
76
200
EvaGreen
RG
10
100
100
Differentiation of Solenopsis invicta social forms using high resolution..
Oakey
2011
 
 
GT73
Plant
Oilseed rape
Amp
2
510
5
0
0
RG
0
100
2550
Mutation Scanning in a Single and a Stacked Genetically Modified (GM)..
Ben Ali
2014
 
 
Ha1-Ha6
Parasite
F. magna
UL Typing
249
3
122
439
LCGreen Plus
LS
7
100
249
Development of high-resolution melting (HRM) analysis for population..
Radvansky
2011
 
 
HASPB
Parasite
Leishmania..
Amp
2
63
3
290
650
EvaGreen
RG
63
100
189
Polymorphism in the HASPB Repeat Region of East African Leishmania..
Zackay
2013
 
 
HIS 3, EF3,..
Fungi
Candida..
Amp
2
9
3
121
153
ResoLight
LC480
18
89
9
Analysis of strain relatedness using High Resolution Melting in a case of..
Gago
2013
 
 
HSP70 and ITS1
Parasite
Leishmania..
Amp
2
35
2
0
0
SYTO 9
ABI 7500
0
100
70
Identification of Six New World Leishmania species through the..
Hernández
2014
 
 
HSP70, VTG,..
Fish
Red abalone..
Amp
2
40
7
80
200
EvaGreen
Eco
0
0
0
SNP discovery and High Resolution Melting Analysis from massive..
2013
 
 
Hsp70h
Plant
Grapevine
Amp
2
169
6
167
305
SYTO 9
RG
4
0
0
Real-time RT-PCR high-resolution melting curve analysis and multiplex..
Bester
2012
 
 
HSP90
Bacteria
Erwinia..
Amp
2
4
1
0
0
SYBR Green I
LC480
4
0
0
Putative resistance gene markers associated with quantitative trait loci..
Gardiner
2012
 
 
HY1, HY2, HY3,..
Plant
A. thaliana
Amp Typing
100
8
0
100
ResoLight
LC480
22
0
0
Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis..
Kazama
2011
 
 
IGF-1
Animal
Cow
Amp
2
208
0
0
0
EvaGreen, SYBR..
RG
0
100
0
SNP exploring in the middle and terminal regions of the IGF-1 gene and..
Abdolmohammadi
2014
 
 
IGS1
Fungi
Candida..
Amp
2
33
1
0
0
EvaGreen
CFX96
0
100
33
Ribosomic DNA intergenic spacer 1 region is useful when identifying..
Gago
2014
 
 
ITS, RAPD
Plant
V. officinalis
Amp Typing
2
23
46
0
0
?
BEBO
13
0
0
Identification of Verbena officinalis based on ITS sequence analysis and..
Ruzicka
2009
 
 
ITS, SLX1, HR,..
Parasite
B. malayi
Scanning
2
5
5
280
1641
SYTO 9
RG
5
100
5
High resolution melting real-time PCR for rapid discrimination between..
Areekit
2009
 
 
ITS-2
Parasite
E. acervulina,..
Amp Typing
?
46
3
340
560
SYTO 9
RG
7
100
21
A polymerase chain reaction-coupled high-resolution melting curve..
Kirkpatrick
2009
 
 
ITS-2
Parasite
Necator..
Amp
2
58
1
0
0
SYTO 9
ABI 7500
5
100
58
Rapid Detection and Identification of Human Hookworm Infections through..
Ngui
2012
 
 
ITS1
Parasite
Leishmania..
Amp
2
1
2
0
0
0
0
0
Mucocutaneous Leishmania tropica infection in a dog from a human cutaneous..
Baneth
2014
 
 
ITS1
Parasite
Leishmania
Amp
2
104
1
350
350
SYBR Green I
RG
0
100
104
Comparison of Clinical Samples and Methods in Chronic Cutaneous..
Eroglu
2014
 
 
ITS1 rDNA
Parasite
Leishmania
Amp Typing
2
300
1
265
288
SYTO 9
RG
4
100
300
Detection and identification of old world Leishmania by high resolution..
Talmi-Frank
2010
 
 
ITS1 rRNA
Parasite
Leishmania
Amp Typing
2
300
1
265
288
SYTO 9
RG
5
100
300
Detection and identification of old world Leishmania by high resolution..
Talmi-Frank
2010
 
 
ITS1, 5.8S rDNA
Fungi
Aspergillus
Mult Det
2
4
4
83
104
LCGreen Plus
HR-1
4
0
0
Multiplex single-color PCR with amplicon melting analysis for..
Erali
2006
 
 
ITS1-ITS2 rDNA
Fungi
Candida
Amp Typing
2
32
1
550
900
ResoLight
LC480
8
0
0
High-throughput identification and quantification of candida species using..
Mandviwala
2010
 
 
ITS2
Fungi
Various Candida
Amp
1,2
46
46
200
500
EvaGreen
CFX96
16
0
0
Detection of Invasive Fungal Pathogens by Real-time PCR and..
Somogyvari
2012
 
 
ITS2
Plant
Sideritis
Detection
2
22
1
0
0
SYTO 9
RG
0
100
22
DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for..
Kalivas
2014
 
 
ITS2 rDNA
Parasite
Crypto
Amp Typing
1, 2
143
1
440
450
SYTO 9
RG
3
100
11
High resolution melting-curve (HRM) analysis for the diagnosis of..
Pangasa
2008
 
 
ITS2 rDNA
Insect
An funestus
Mult Det
2
96
5
146
587
SYBR Green I
RG
5
93.6
94
Development of multiplex real-time PCR assays for identification of..
Vezenegho
2009
 
 
ITS2 rDNA
Fungi
Candida
Amp Typing
2
22
1
141
302
ResoLight
LC480
8
95
22
High-throughput identification and quantification of candida species using..
Mandviwala
2010
 
 
JAK1 and STAT3
Animal
Rabbit
Amp
2
253
3
120
172
EvaGreen
CFX96
0
100
759
Investigation of JAK1 and STAT3 polymorphisms and their gene–gene..
Fu
2014
 
 
kdr
Insect
An gambiae
Amp Typing
2
96
1
71
71
SYTO 9 (1.5 uM)
RG
5
91.3
80
Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a..
Bass
2007
 
 
ldh-A, mlc-2,..
Fish
Swordfish,..
Amp, UL
2
121
3
0
0
LCGreen Plus
0
0
0
High-resolution melting analysis (HRMA): a highly sensitive inexpensive..
Smith
2010
 
 
ldlr, nsd2
Fish
Zebrafish
Detection
2
0
0
0
0
SYBR Green I
CFX96
0
0
0
A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and..
Yu
2014
 
 
LF, TLR4
Animal
Cow
Amp
2
167
3
103
137
EvaGreen
RG
4
0
0
Single nucleotide polymorphisms in immunity-related genes and their..
Carvajal
2013
 
 
Lr14a
Plant
Durum wheat..
Detection
2
176
6
56
59
SYTO 9
ABI 7500
0
0
0
Development of COS-SNP and HRM markers for high-throughput and reliable..
Terracciano
2013
 
 
Ly49 and class..
Animal
Mice
Detection
2
233
7
63
113
EvaGreen
CFX96
2
100
1631
Rapid discrimination of MHC class I and killer cell lectin-like receptor..
Lundgren
2012
 
 
MACPPE24
Bacteria
Amp
2
10
0
0
0
0
0
0
PCR amplification and high-resolution melting curve analysis as a rapid..
Castellanos
2010
 
 
matK
Plant
H. niger
Amp Typing
7
3
116
333
EvaGreen
RG
8
100
7
Quantitative high-resolution melting analysis for detecting adulterations
Mader
2011
 
 
matK
Plant
H. niger
Amp Typing
98
9
0
0
BEBO
RG
12
100
98
DNA-based identification of Helleborus niger by high-resolution melting..
Schmiderer
2010
 
 
mdr1
Parasite
Plasmodium..
Amp Typing
2
58
1
173
173
EvaGreen
RG
2
78
58
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
MEKK1, MEKK2,..
Plant
A. thaliana
Amp Typing
294
10
99
124
S13
CFX96
12
0
0
iTILLING: A Personalized Approach to the Identification of Induced..
Bush
2010
 
 
MP65
Fungi
Nomen..
Amp
5
3
476
476
LCGreen Plus
LC2.0
5
100
15
Rapid, simple, and low-cost identification of Candida species using..
Arancia
2011
 
 
Mstn
Fish
Takifugu..
Nested PCR
2
384
3
268
586
SYTO 9
LC480
10
100
1152
New approach for fish breeding by chemical mutagenesis: establishment of..
Kuroyanagi
2013
 
 
MSTN, GDF8
Animal
Cattle
Detection
2
1140
0
0
0
SYTO 9
RG
15
100
0
Real-time PCR genotyping and frequency of the myostatin F94L mutation in..
Vankan
2010
 
 
mt SSU rRNA
Plant
D. pinea, D...
Amp Typing
30
1
89
89
EvaGreen
RG
5
100
30
High-resolution melting analysis: a new molecular approach for the early..
Luchi
2011
 
 
mtDNA
Animal
B. primigenius
Amp Typing
75
3
74
114
LCGreen Plus
HR-1 and LS
5
100
74
Application of high-resolution melting for genotyping bovine mitochondiral..
Zhou
2011
 
 
ND4
Fish
Sharks -..
Amp
2
160
3
0
0
EvaGreen
RG
0
100
480
A mitochondrial species identification assay for Australian blacktip..
Morgan
2011
 
 
ND6 and ND4
Animal
Mice
Amp
2
22
19
109
218
LCGreen Plus
LS
19
0
0
The identification of mitochondrial DNA variants in glioblastoma multiforme
Yeung
2014
 
 
Net2
Plant
Aegilops..
Detection
2
120
12
93
145
ResoLight
LC480
4
0
0
Application of real-time PCR-based SNP detection for mapping of Net2, a..
Matsuda
2012
 
 
OBHRM
Rodent
Lepob
Amp
2
0
1
61
61
ResoLight
LC480
0
0
0
Efficient Method of Genotyping Ob/Ob Mice Using High Resolution Melting..
Sutter
2013
 
 
Ocp
Animal
Ochotona..
Amp
2
42
85
143
224
EvaGreen
CFX96
1
0
0
Novel genomic resources for a climate change sensitive mammal:..
Lemay
2013
 
 
oxyR
Animal
Cows, goats
Amp
2
208
2
60
79
SYTO 9
RG
2
100
416
First-Time Detection of Mycobacterium bovis in Livestock Tissues and Milk..
Ereqat
2013
 
 
p53
Fish
Zebrafish
Amp Typing
2
48
1
39
39
LCGreen Plus
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
p53
Fish
zebrafish and..
Amp
2
0
4
0
0
LS
0
0
0
High-resolution melting curve analysis for rapid detection of mutations in..
Ishikawa
2010
 
 
p53
Fish
Oryzias latipes
Detection
2
400
0
0
0
LS, LC
0
100
0
p53-Dependent suppression of genome instability in germ cells
Otozai
2014
 
 
PAL, P5CS and..
Plant
Rosa
Detection
2
3
3
204
227
ResoLight
LC480
0
0
0
Anchoring Linkage Groups of the Rosa Genetic Map to Physical Chromosomes..
Kirov
2014
 
 
PepMV
Virus
Pepino mosaic..
Amp
2
12
2
88
128
ResoLight
LC480
2
0
0
A new method for detection and discrimination of Pepino mosaic virus..
Hasiów-Jaroszew..
2013
 
 
pfcrt
Parasite
Plasmodium..
Amp
2
53
2
0
0
LCGreen Plus
LS
0
96.2
51
Rapid Identification of chloroquine and atovaquone drug resistance in..
Gan
2010
 
 
pfcrt, pfdhfr,..
Parasite
Plasmodium..
UL
1
66
23
0
0
LCGreen Plus
LS96
6
100
1518
Rapid, Field-Deployable Method for Genotyping and Discovery of..
Daniels
2012
 
 
Pfdhfr
Parasite
P. falciparum
UL Typing
4
4
641
641
LCGreen Plus
LC480
4
100
4
High-Throughput Genotyping of Single Nucleotide Polymorphisms in the..
Cruz
2010
 
 
phyA
Plant
Olea europaea
Amp
2
22
4
307
307
LCGreen Plus
HR-1
6
0
0
Mutation scanning and genotyping by high-resolution DNA melting analysis..
Muleo
2009
 
 
PLIN2
Animal
S. scrofa
Amp Typing
32
7
244
398
SYBR Green I
LC480
6
100
32
New SNP of the porcine perilipin 2 (PLIN2) gene, association with carcass..
Davoli
2011
 
 
pmr
Plant
Sweet Cherry..
Amp
2
114
40
0
0
ResoLight
LC480
2
0
0
Genetic diversity and relatedness of sweet cherry (prunus avium L.)..
Fernandez
2012
 
 
pol
Animal
Pig
Nested
2
111
4
101
242
ResoLight
LC480
0
0
0
Multiplex high resolution melting assay for estimation of Porcine..
Wynyard
2011
 
 
pol
Animal
Pig
2
14
4
101
242
ResoLight
LC480
0
0
0
Microbiological safety of the first clinical pig islet xenotransplantation..
Wynyard
2014
 
 
PpAG, PpTFL1
Plant
P. persica
Scanning
36
6
131
149
ResoLight
LC480
12
100
36
Mutation scanning of peach floral genes
Chen
2011
 
 
PPIB
Animal
American..
Detection
2
690
1
81
81
SYTO 9
ABI 7500
4
100
690
Allele frequency of hereditary equine regional dermal asthenia in American..
Badial
2013
 
 
rap-1c
Parasite
Babesia..
Amp
2
5
0
0
0
EvaGreen
RG
0
0
0
Typification of virulent and low virulence Babesia bigemina clones by 18S..
Thompson
2014
 
 
RPO30
Virus
Capripoxvirus
Amp
2
63
1
96
96
EvaGreen
CFX96
3
100
63
Development of a Cost-Effective Method for Capripoxvirus Genotyping Using..
Gelaye
2013
 
 
rpoB
Bacteria
Geobacillus
Amp
2
46
7
0
0
SYBR Green I
RG
0
0
0
Genotyping of Present-Day and Historical Geobacillus Species Isolates from..
Seale
2012
 
 
rpoB
Animal, Parasite
Harbor seal,..
Detection
2
48
4
0
0
SYTO 9
RG
0
0
0
Identification of a Bartonella species in the harbor seal (Phoca vitulina)..
Morick D
2009
 
 
rpoB, gltA,..
Parasite
eight..
Amp Typing
2
112
3
191
360
SYTO 9
RG
10
100
336
Detection of Bartonella spp. in wild rodents in Israel using HRM real-time..
Morick
2009
 
 
rps16
Plant
Arenaria..
Amp
2
380
2
360
390
SYBR Green I
LC480
2
99
379
Rapid identification of chloroplast haplotypes using High Resolution..
Dang
2012
 
 
rRNA
Fungus
Fusarium..
UL
2
7
1
0
0
SYTO 9
RG
7
100
7
High-resolution melting analysis allowed fast and accurate closed-tube..
Ganopoulos
2012
 
 
Ruh.7H
Plant
Barley
Detection
2
1
13
90
340
SYTO 9
RG
0
0
0
The use of high resolution melting (HRM) to map single nucleotide..
Lehmensiek
2008
 
 
SBEIIa-A
Plant
Amp Typing
56
12
152
313
LCGreen Plus
LS
6
0
0
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
SBEIIa-B
Plant
Amp Typing
14
6
19
204
LCGreen Plus
LS
6
100
14
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
SBEIIa-D
Plant
Amp Typing
53
9
151
301
LCGreen Plus
LS
6
0
0
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
sdY
Fish
Salmo salar..
Amp
2
34
2
0
0
EvaGreen
CFX96
0
0
0
A rapid qPCR method for genetic sex identification of Salmo salar and..
d’Auriac
2014
 
 
see note
Plant
P. dulcis
Amp Typing
93
12
68
247
SYTO 9
RG
12
100
93
Mapping SNP-anchored genes using high-resolution melting analysis in almond
Wu
2009
 
 
shsp
Insect
Drosophila..
Amp
2
0
1
100
100
ResoLight
LC480
0
0
0
Characterization of the shsp genes in Drosophila buzzatii and association..
Frydenberg
2010
 
 
SL-IR,..
Parasite
Trypanosoma..
Amp
2
38
2
0
0
SYTO 9
ABI 7500
38
50
76
Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) through..
Higuera
2013
 
 
SNP markers
Fish
O. keta
Amp Typing
190
10
118
412
BigDye
AB
3
100
95
Transcriptome sequencing and high-resolution melt analysis advance single..
Seeb
2011
 
 
SNP markers
Plant
A. sativa
Amp Typing
6
8
4
60
120
EvaGreen
CFX96
4
0
0
Model SNP development for complex genomes based on hexaploid oat using..
Oliver
2011
 
 
SNP markers
Plant
Apple
Amp Typing
2, 3, 4
6
167
0
300
LC480
0
0
0
Development of a set of SNP markers present in expressed genes of the apple
Chagne
2008
 
 
SNP markers
Plant
M. domesctica
Amp Typing
14
1
300
550
LC480
6
100
14
Development of a set of SNP markers present in expressed genes of the apple
Chagne
2008
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
167
167
SYTO 9
RG
0
97.1
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
210
210
SYTO 9
RG
0
93.6
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
235
235
SYTO 9
RG
0
92.1
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSR markers
Plant
Grape
Amp Typing
2, 4
14
9
129
315
ResoLight
LC480
6
0
0
A new approach to varietal identification in plants by microsatellite high..
Mackay
2008
 
 
SSR markers
Plant
Olive
Amp Typing
2, 3, 4
2
3
0
0
ResoLight
LC480
2
0
0
A new approach to varietal identification in plants by microsatellite high..
Mackay
2008
 
 
SSR214
Plant
Oregano
Amp Typing
2
82
1
92
92
BEBO
RG
5
100
8
A strategy to setup codominant microsatellite analysis for..
Mader
2008
 
 
SSR244
Plant
Oregano
Amp Typing
2
82
1
148
148
BEBO
RG
10
100
12
A strategy to setup codominant microsatellite analysis for..
Mader
2008
 
 
SSU rDNA
Parasite
Di fragilis
Amp Typing
2
30
1
662
662
SYBR Green I
LC480
4
0
0
Genetic diversity of Dientamoeba fragilis of irritable bowel syndrome..
Hussein
2009
 
 
ssu-rRNA, bg,..
Parasite
Giardia..
Amp
2
40
2
0
0
SYBR Green I
LC480
0
0
0
Genotypes of Giardia intestinalis clinical isolates of gastrointestinal..
Al-Mohammed
2011
 
 
STS markers
Plant
White lupin
Amp Typing
2, 4, 8
2
77
91
391
SYTO 9
RG
0
0
0
High resolution melt analysis to identify and map sequence-tagged site..
Croxford
2008
 
 
t610, t208
Bacteria
Daphnia magna
Amp
2
15
2
100
200
EvaGreen
LC480
15
100
30
High-resolution melting analysis: a genotyping tool for population studies..
Kuster
2012
 
 
TBC1D1
Animal
S. scrofa
Amp Typing
778
3
32
0
EvaGreen
RG
5
100
9
The porcine TBC1D1 gene: mapping, SNP identification, and association..
Fontanesi
2011
 
 
tpi
Parasite
Giardia..
Amp
2
11
1
334
334
EvaGreen
RG
2
100
11
Application of HRM assays with EvaGreen dye for genotyping Giardia..
Zhang
2012
 
 
Tpi
Insect
Ostrinia..
UL, Amp
2
30
3
95
142
LCGreen Plus
HR-1
2
100
90
Application of High-Resolution DNA Melting for Genotyping in Lepidopteran..
Li
2012
 
 
trnL
Plant
Pinus spp.
Amp
2
74
3
0
0
SYTO 9
RG
9
100
222
Taxonomic Identification of Mediterranean Pines and Their Hybrids Based on..
Ganopoulos
2013
 
 
trnL and rpoC1
Plant
Various oils
Amp
2
18
2
0
0
6
0
0
Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel..
Ganopoulos
2013
 
 
trnL-trnF
Plant
H. niger
Amp Typing
98
6
0
0
BEBO
RG
15
100
98
DNA-based identification of Helleborus niger by high-resolution melting..
Schmiderer
2010
 
 
Tvr1
Plant
Lettuce
Scanning
2
192
5
157
648
LCGreen Plus
LS
0
0
0
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
UBE2
Animal
Concholepas..
Amp
2
18
1
0
0
SYBR Green I
Eco
1
0
0
Ubiquitin-conjugating enzyme E2-like gene associated to pathogen response..
Núñez-Acuña
2012
 
 
UDA
Plant
Urtica dioica
Detection
2
12
2
297
463
EvaGreen
RG
11
0
0
RAPD-PCR and real-time PCR HRM BASED GENETIC VARIATION EVALUATIONS of..
Uzonur
2013
 
 
Various
Fish
Zebrafish..
Detection
2
47
101
50
300
LCGreen Plus
LS-96
0
0
0
High-Throughput Genome Editing and Phenotyping Facilitated by High..
Thomas
2014
 
 
vip3
Bacteria
Bacillus..
Amp
2
11
1
0
0
LCGreen Plus
LS
0
0
0
Detection and Identification of Vegetative Insecticidal Proteins vip3..
Li
2012
 
 
Vssc
Insect
S. scabiei
Amp Typing
2
249
7
144
144
SYTO 9
RG
4
100
20
High-resolution melt analysis for the detection of a mutation associated..
Pasay
2008
 
 
VvDXS
Plant
Vitis vinifera..
Scanning
2
242
2
125
147
LCGreen Plus
LC480
3
0
484
Development of user-friendly functional molecular markers for VvDXS gene..
Emanuelli
2013
 
 
VvNCED1
Plant
Grapevine
Detection
2
24
9
125
155
ResoLight
LC480
2
100
216
EMS mutagenesis and qPCR-HRM prescreening for point mutations in an..
Acanda
2013
 
 
Waxy
Plant
C. annuum, C...
Amp Typing
31
8
110
457
SYTO 9
RG
6
100
31
Identification of Capsicum species using SNP markers based on high..
Jeong
2010
 
 
wdr43
Fish
Zebrafish
Amp Typing
2
48
1
50
50
LCGreen Plus
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
XTH
Plant
G. hirsutum
Amp Typing
2
6
1
195
195
SYTO 9
RG
2
100
6
Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from..
Michailidis
2009