NonHuman Polyploid Organisms

Gene
Order
Species
Method
Ploidy
# Samp
Amplicons
Dye
Inst
# Variants
Accuracy
Title
Author
Year
Link
Email
Length
#
Min
Max
%
#
Plant
P. dulcis
Scanning
2
25
4
68
198
S9
RG
17
100
25
High resolution melting analysis of almond SNPs derived from ESTs
Wu
2008
 
 
Plant
M. sativa
Scanning
4
191
2
40
200
LCG+
LS
2
87
13
Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and..
Han
2011
 
 
Parasite
Clonorchis..
Amp
2
14
1
165
165
HRM
LC480
1
100
14
Molecular Differentiation of Opisthorchis viverrini and Clonorchis..
Kaewkong
2013
 
 
Parasite
Schistosoma..
Detection
2
0
2
156
156
RL
LC480
2
0
0
Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi..
Kongklieng
2013
 
 
Parasite
Wuchereria..
Amp
2
14
1
161
227
RL
LC480
4
100
14
Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi,..
Thanchomnang
2013
 
 
Parasite
Babesia canis
Amp Typing
2
800
2
0
0
SG1
RG
7
100
158
The factors affecting the distribution of babesiosis in dogs in Poland
Adaszek
2011
 
 
Parasite
Plasmodium..
Amp
2
88
1
165
165
EG
CFX96
2
98
86
Rapid Discrimination between Anopheles gambiae s.s. and Anopheles..
Zianni
2013
 
 
Parasite
Opisthorchis..
Amp
2
7
1
209
209
RL
LC480
1
100
7
Molecular Differentiation of Opisthorchis viverrini and Clonorchis..
Kaewkong
2013
 
 
Plant
Prototheca..
Amp
10
1
250
250
SG
RG
1
100
10
Rapid Molecular Typing of Prototheca zopfii by High Resolution Melting..
Sobukawa
2013
 
 
Parasite
Filarial..
Detection
1, 2
2039
1
115
115
RL
LC480
3
0
0
A high resolution melting real time PCR for mapping of filaria infection..
Wongkamchai
2013
 
 
Animal
Mollusca,..
UL
2
6
3042
100
100
LCG+
LS
1
0
0
Large-Scale Development of Gene-Associated Single-Nucleotide..
Jiao
2013
 
 
Parasite
D. immitis, D...
Amp
2
15
1
256
256
EG
Eco
2
100
15
Rapid differentiation of Dirofilaria immitis and Dirofilariarepens in..
Albonico
2013
 
 
Plant
Plant Oil
Detection
2
9
1
71
71
S9
7900HT
9
0
0
Detection of plant oil DNA using high resolution melting (HRM) post PCR..
Vietina
2013
 
 
Fungi
(Sporothrix..
Amp
1, 2
455
1
0
0
SG
RG
3
0
0
High-resolution DNA melt-curve analysis for cost-effective mass screening..
McCarthy
2013
 
 
Fish
Kokanee salmon
Amp
2
88
36
68
171
EG
CFX96
0
0
0
Transcriptome-wide comparison of sequence variation in divergent ecotypes..
Lemay
2013
 
 
Plant
Alfalfa
Amp
2
192
20
51
170
LCG+
LS
0
0
0
Prevalence of single nucleotide polymorphism among 27 diverse alfalfa..
Li
2012
 
 
Plant
Citrus
Amp
2
15
24
0
0
S9
RG
0
100
360
High Resolution Melting Analysis Is a More Sensitive and Effective..
Distefano
2012
 
 
Plant
Prunus avium..
Detection
2
8
223
0
0
SG1
LC480
8
0
0
Rapid gene-based SNP and haplotype marker development in non-model..
Koepke
2012
 
 
Fungi
Acanthamoeba..
Amp
38
2
204
210
S9
ABI 7500
10
70
53
New Strategy for Rapid Diagnosis and Characterization of Keratomycosis
Goldschmidt
2012
 
 
Fish
Pacific..
Amp
2
14
14
0
0
RL
LC480
0
0
0
Development of Genomic Resources for Pacific Herring through Targeted..
Roberts
2012
 
 
Parasite
Leishmania..
2
208
2
265
273
0
0
0
Leishmania tropica Infection in Golden Jackals and Red Foxes, Israel
Talmi-Frank
2010
 
 
Plant
Arabidopsis..
Amp
2
0
3
0
0
RG
0
0
0
A simple, high throughput method to locate single copy sequences from..
Vu
2010
 
 
Plant
Potato,..
Amp
2
0
7
100
300
LCG+
LS
0
0
0
A High-Resolution Melting Approach for Analyzing Allelic Expression..
Yuan
2009
 
 
Insect
Drosophila..
2
0
2
0
0
LCG+
LS
0
0
0
CRISPR/Cas9 mediated genome engineering in Drosophila
Bassett
2014
 
 
Fungi
Eight Candida..
Amp
2
19
2
0
0
S9
Eco
0
0
0
The development and validation of a rapid genetic method for species..
Alnuaimi
2014
 
 
Plant
Pummelo..
Amp
2
260
25
35
61
LCG+
LS
0
0
0
Identification of Pummelo Cultivars by Using a Panel of 25 Selected SNPs..
Wu
2014
 
 
Animal
Sheep
Amp
2
24
4
0
0
LCG+
LS
0
0
0
Limitation of high-resolution melting curve analysis for genotyping simple..
Yang
2014
 
 
12S and 18S RNA
Animal
buffalo,..
Detection
2
30
2
77
220
S9
RG
5
0
0
A fast and accurate method for controlling the correct labeling of..
Sakaridis
2013
 
 
12S rRNA
Parasite
B. malayi, B...
Amp
2
48
1
115
115
RL
LC480
3
100
48
Rapid Detection and Identification of Brugia malayi, B. pahangi, and..
Wongkamchai
2013
 
 
12S-rRNA
Animal
10 species
Amp
2
137
10
93
399
EG
RG
0
100
137
Reliable Discrimination of 10 Ungulate Species Using High Resolution..
Ramon-Laca
2014
 
 
139 genes
Animal
Bulls
Detection
2
42
407
0
0
S9
ABI 7500
1613
0
0
High-resolution melt analysis does not reveal mutagenic risk in sexed..
Pozzi
2014
 
 
159 genes
Plant
Pea
Amp
2
2
0
0
0
RL
RG
2
0
0
Combining gene expression and genetic analyses to identify candidate genes..
Legrand
2013
 
 
16S rDNA
Bacteria
Bacillus..
Amp
1, 2
237
2
70
100
EG
RG
7
100
474
Rapid identification of dairy mesophilic and thermophilic sporeforming..
Chauhan
2013
 
 
16S rRNA
Bacteria
Anaplasma..
Amp
2
42
1
257
257
EG
Biorad
2
100
42
A Novel High-Resolution Melt PCR Assay Discriminates Anaplasma..
Krucken
2013
 
 
16S rRNA
Bacteria
Various
Detection
70
3
0
0
LCG+
HR-96
0
90
63
Comparative Analysis of Two Broad-Range PCR Assays for Pathogen Detection..
Jeng
2012
 
 
16S rRNA
Bacteria
Lactic Acid..
Amp
Various
86
1
0
0
RL
LC480
13
0
0
Strain-level characterization of nonstarter lactic acid bacteria in..
Porcellato
2012
 
 
16S rRNA
Bacteria
Various
Detection
106
3
0
0
LCG+
LS
21
70.6
75
Identification of Bacterial Pathogens in Ascitic Fluids from Patients with..
Hardick
2012
 
 
16S rRNA
Bacteria
Various
Detection
21
2
150
200
RL
LC480
0
0
0
Combined Molecular Gram Typing and High- Resolution Melting Analysis for..
Ozbak
2012
 
 
16S rRNA
Bacteria
Various
Amp
13
2
0
0
RL
LC480
13
77
10
Rapid lactic acid bacteria identification in dairy products by..
Porcellato
2012
 
 
16S rRNA
Bacteria, Fungi
Various
0
0
0
0
S9, LCG+, SG1,..
HR-1, RG, LC, LS
0
0
0
The potential of high resolution melting analysis (hrma) to streamline,..
Ruskova
2011
 
 
16S rRNA,..
Algae
cyanobacterial..
Detection
2
206
0
0
0
RG
5
100
0
Rapid, multiplex-tandem PCR assay for automated detection and..
Baker
2013
 
 
18S
Parasite
Babesia canis..
Amp Typing
2
20
1
559
559
SG1
2
0
0
Application of the SYBR Green real-time HRM PCR technique in the..
Adaszek
2010
 
 
18S
Bacteria, Fungi
Staphylococcus..
Amp
1, 2
8
2
180
210
S9
ABI 7500
0
0
0
Enzymatic Treatment of Specimens before DNA Extraction Directly Influences..
Goldschmidt
2014
 
 
18S rDNA
Fungus
Mucormycosis
Amp
31
0
0
0
EG
RG
0
0
0
Rapid Detection and Identification of Mucormycetes from Culture and Tissue..
Hrncirova
2010
 
 
18S rRNA
Fungi
Various
Amp
10
2
162
270
S9
ABI 7500
12
0
0
New Strategy for Rapid Diagnosis and Characterization of Fungal..
Goldschmidt
2012
 
 
18S rRNA
Parasite
Babesia..
Detection
2
299
0
0
0
0
0
0
Frequency and therapy monitoring of canine Babesia spp. infection by..
Wang
2010
 
 
26S/28S lsrDNA
Parasite
Trichinella
Amp
2
4
1
90
90
EG
RG
4
100
4
Ribo HRM — Detection of inter- and intra-species polymorphisms within..
Masny
2012
 
 
6msas and idh
Fungi
Penicillium..
Amp
2
21
1
96
96
EG
CFX96
21
100
21
Genetic structure and natural variation associated with host of origin in..
Sanzani
2013
 
 
7SL RNA
Parasite
Leishmania
Amp Typing
2
269
1
119
119
1.5 uM S9
RG
4
100
269
Rapid diagnosis of old world Leishmaniasis by high resolution melting..
Nasereddin
2010
 
 
7SL RNA
Parasite
Leishmania..
Amp
2
13
2
0
0
EG
CFX96
0
0
0
Natural Infection of North African Gundi (Ctenodactylus gundi) by..
Bousslimi
2012
 
 
897 genes
Mollusc
Oyster
Amp
2
60
1671
39
140
LCG+
HR-96
0
0
0
Discovery and validation of genic single nucleotide polymorphisms in the..
Wang
2015
 
 
ace-2
Insect
Culex pipiens
Amp
2
60
1
76
76
EG
RG
3
97
58
Identification of Culex complex species using SNP markers based on..
Kang
2013
 
 
apc
Fish
Zebrafish
Amp Typing
2
48
1
53
53
LCG+
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
Apj
Animal
Sea Cucumber..
UL
2
159
200
70
110
LCG+
HR-96
48
0
0
Development of 101 Gene-based Single Nucleotide Polymorphism Markers in..
Du
2012
 
 
ARF7, Exp,..
Plant
S. lycopersicum
Amp Typing
2
22742
5
220
469
LCG+
LS
3
99.3
1640
Implementation of two high through-put techniques in a novel application:..
Gady
2009
 
 
ARP, ANT,..
Fish
swordfish..
UL
2
774
10
300
950
LCG+, EG
LC480
2
100
7740
Methodological streamlining of SNP discovery and genotyping via..
Smith
2013
 
 
At5G49430,..
Plant
Medicago..
Amp
2
6
20
100
150
LCG+
ABI 7500
2
100
120
High-resolution melting analysis for SNP genotyping and mapping in..
Han
2012
 
 
B1
Parasite
Toxoplasma..
Amp Typing
2
14
3
82
481
LCG+
LC480
3
100
42
Genotyping of the protozoan pathogen Toxoplasma gondii using..
Costa
2011
 
 
B1
Parasite
Toxoplasma..
Amp Typing
2
14
3
82
481
LCG+
LC480
3
100
42
High-resolution melting for analysis of short sequence repeats in..
Ricchi
2011
 
 
bags13d21
Plant
Barley
Amp Typing
2
8
1
142
142
SG1
RG
0
100
8
Temperature Switch PCR (TSP): Robust assay design for reliable..
Tabone
2009
 
 
BAL
Fungi
Rhizopus spp.,..
Detection
2
99
0
0
0
RG
5
0
0
Rapid Detection and Identification of Mucormycetes in Bronchoalveolar..
Lengerova
2014
 
 
bap28
Fish
Zebrafish
Amp Typing
2
48
1
41
46
LCG+
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
Bbaa2
Animal
Mice
UL
2
0
2
0
0
LCG+
LC480
0
0
0
High-throughput genotyping of advanced congenic lines by High Resolution..
Bramwell
2012
 
 
BraA.CAX1a and..
Plant
Brassica rapa.
Amp
2
0
4
0
0
EG
LS
37
100
0
High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS..
Lochlainn
2011
 
 
C1H21orf62,..
Animal
Datong Yak
Amp
2
101
9
60
100
LCG+
LS
2
0
0
Associations of single nucleotide polymorphisms in candidate genes with..
Liu
2013
 
 
C3'H, C4H,..
Plant
C. cardunculus
Amp Typing
192
3
63
627
EG
RG
4
0
0
Genetic map of artichoke x wild cardoon: toward a consensus map for Cynara..
Sonnante
2011
 
 
C9orf72, Fus
Rodent
Mice
Detection
2
10
2
133
140
LCG+
LS
2
100
20
Highly Efficient Targeted Mutagenesis in Mice Using TALENs
Panda
2013
 
 
CDC3
Fungi
Ca albicans
Amp Typing
2
95
1
94
110
RL
LC480
0
0
0
Genotyping of Candida albicans using length fragment and high-resolution..
Costa
2010
 
 
CDC3
Fungi
yeast
Amp Typing
2
95
1
0
0
RL
LC480
3
0
0
Genotyping of Candida albicans using length fragment and high-resolution..
Costa
2010
 
 
CDC3
Fungi
Candida..
Amp
2
82
1
0
0
LC480
0
0
0
Temporal Similarity Between Candida albicans Genotypes in a Tunisian..
Abdeljelil
2012
 
 
CG14073
Insect
Drosophila
Detection
2
0
0
0
0
LCG+
LS
2
0
0
Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9..
Bassett
2013
 
 
CHD1
Animal
Bird
Amp
2
111
1
2
44
EG
CFX96
14
0
0
High-resolution melting analysis for bird sexing: a successful approach to..
Morinha
2013
 
 
Chloroplast..
Plant
Thale Cress
Scanning
2
1
320
350
1330
LCG+
LC480
0
0
0
A rapid high-throughput method for the detection and quantification of RNA..
Chateigner-Bout..
2007
 
 
Cntg10192
Plant
Lettuce
Amp Typing
2
132
1
185
185
LCG+
LS
0
100
132
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
Cntg4252
Plant
Lettuce
UL Typing
2
192
1
208
208
LCG+
LS
0
0
0
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
CO1
Parasite
Schistosoma..
Detection
2
166
1
0
0
RL
LC480
2
100
166
Natural Interactions between S. haematobium and S. guineensis in the..
Mone
2012
 
 
COI
Insect
L. sericata,..
Amp Typing
15
15
70
119
EG
RG
15
100
15
Identification of forensically important blowfly species (Diptera:..
Malewski
2010
 
 
COI
Insect
Drosophila..
Amp
2
65
1
150
150
EG
CFX96
0
0
0
A HRM Real-Time PCR Assay for Rapid and Specific Identification of the..
Dhami
2014
 
 
Col-0, Ler-0
Plant
2
4
0
0
0
LC480
0
0
0
Screening for Imprinted Genes Using High-Resolution Melting Analysis of..
Day
2014
 
 
cox1
Parasite
Echinococcus
Detection
2
107
1
444
444
S13
RG
7
100
107
Rapid detection of Echinococcus species by a high-resolution melting (HRM)..
Santos
2013
 
 
cox1
Parasite
Fascioloides..
Detection
2
200
1
439
439
LCG+
LS
2
0
0
The origin of the giant liver fluke, Fascioloides magna (Trematoda:..
Bazsalovicsová
2013
 
 
Cre
Rodent
Mice
Detection
2
25
2
82
700
LCG+
5
0
0
High Resolution Melt Analysis (HRMA); a Viable Alternative to Agarose Gel..
Thomsen
2012
 
 
crt
Parasite
Plasmodium..
UP
2
53
1
145
145
LCG+
LS
4
100
53
Rapid identification of choloroquine and atovaquone drug resistance in..
Gan
2010
 
 
crt
Parasite
Plasmodium..
Amp Typing
2
59
1
198
198
EG
RG
2
100
59
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
cry9Da4,..
Bacteria
Bacillus..
Amp
2
48
1
100
100
LCG+
LS
48
0
0
Characterization of cry9Da4, cry9Eb2, and cry9Ee1 genes from Bacillus..
Shu
2013
 
 
CYP51A
Fungi
As fumigatus
Amp Typing
2
25
1
107
107
EG
RG
0
100
12
Use of a high-resolution melt assay to characterize codon 54 of the cyp51A..
Tuohy
2010
 
 
cyp51A
Fungi
Aspergillus
UL
2
78
2
354
354
RL
LC480
6
100
156
Rapid Detection and Identification of Aspergillus from Lower Respiratory..
Alonso
2012
 
 
Cyt b
Parasite
Trypanosoma..
Detection
2
36
1
383
383
SG1
RG
14
100
36
High-Resolution Melting (HRM) of the Cytochrome B Gene: A Powerful..
Pena
2012
 
 
cyt bc1
Parasite
Plasmodium..
UP
2
53
1
116
116
LCG+
LS
4
100
53
Rapid identification of choloroquine and atovaquone drug resistance in..
Gan
2010
 
 
dhfr
Parasite
Plasmodium..
Amp Typing
2
203
3
167
200
EG
RG
2
98
203
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
dhps
Parasite
Plasmodium..
Amp Typing
2
87
2
181
195
EG
RG
2
89
87
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
DLOOP
Cheese
Feta
Amp
15
2
77
145
S9
RG
0
0
0
A novel closed-tube method based on high resolution melting (HRM) analysis..
Ganopoulos
2013
 
 
DMD
Rodent
? Mdx
Amp Typing
?
46
1
84
84
S9
RG
2
96
44
A novel and simple method for genotyping the mdx mouse using..
Trebbin
2009
 
 
DREB1, HKT1,..
Plant
Durum Wheat..
Amp
2
48
4
100
100
EG
RG
4
100
192
Identification of SNP Mutations in DREB1, HKT1, and WRKY1 Genes Involved..
Mondini
2012
 
 
eif2b5
Fish
Zebrafish
2
0
0
0
0
0
0
0
Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution..
Xing
2014
 
 
eIF4E
Plant
Barley
Detection
2
643
7
130
620
LCG+
LS
30
100
4501
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid..
Hofinger
2009
 
 
elF4E
Plant
H. vulgare
Amp Typing
2
643
7
130
620
LCG+
LS
10
100
44
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid..
Hofinger
2009
 
 
ESTs..
Plant
Cr japonica
Amp Typing
2
8
574
90
120
EG
LC480
0
0
0
An efficient method for developing SNP markers based on EST data combined..
Ujino-Ihara
2010
 
 
FcCB
Shrimp
Fenneropenaeus..
UL
2
192
3
0
0
LCG+
HR-96
2
0
0
Molecular cloning and characterization of a cathepsin B gene from the..
Li
2013
 
 
FcERK
Shrimp
Fenneropenaeus..
UL
2
192
2
0
0
LCG+
HR96
1
0
0
Identification, cloning and characterization of an extracellular..
Li
2013
 
 
FOXP2
Fish
Zebrafish
Amp
2
25
1
0
0
LCG+
LS-96
1
0
0
Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding
Xing
2012
 
 
Gln1-3
Plant
Maize
Detection
2
8902
4
0
0
LCG+, EG,
LS
3
0
0
A Cost-effective High-resolution Melting Approach using the EvaGreen Dye..
Li
2010
 
 
Glyma16g34070,..
Plant
Soybean
UL
2
334
1
129
129
RL
LC480
0
0
0
Genetic mapping of the powdery mildew resistance gene in soybean PI 567301B
Jun
2012
 
 
golden, ryr3,..
Fish
Zebrafish
Amp
2
1
6
90
120
LCG+
LS
1
0
0
Simple Methods for Generating and Detecting Locus- Specific Mutations..
Dahlem
2012
 
 
Gp-9
Insect
S. invicta
Amp Typing
100
3
76
200
EG
RG
10
100
100
Differentiation of Solenopsis invicta social forms using high resolution..
Oakey
2011
 
 
Ha1-Ha6
Parasite
F. magna
UL Typing
249
3
122
439
LCG+
LS
7
100
249
Development of high-resolution melting (HRM) analysis for population..
Radvansky
2011
 
 
HASPB
Parasite
Leishmania..
Amp
2
63
3
290
650
EG
RG
63
100
189
Polymorphism in the HASPB Repeat Region of East African Leishmania..
Zackay
2013
 
 
HIS 3, EF3,..
Fungi
Candida..
Amp
2
9
3
121
153
RL
LC480
18
89
9
Analysis of strain relatedness using High Resolution Melting in a case of..
Gago
2013
 
 
HSP70, VTG,..
Fish
Red abalone..
Amp
2
40
7
80
200
EG
Eco
0
0
0
SNP discovery and High Resolution Melting Analysis from massive..
2013
 
 
Hsp70h
Plant
Grapevine
Amp
2
169
6
167
305
S9
RG
4
0
0
Real-time RT-PCR high-resolution melting curve analysis and multiplex..
Bester
2012
 
 
HSP90
Bacteria
Erwinia..
Amp
2
4
1
0
0
SG1
LC480
4
0
0
Putative resistance gene markers associated with quantitative trait loci..
Gardiner
2012
 
 
HY1, HY2, HY3,..
Plant
A. thaliana
Amp Typing
100
8
0
100
RL
LC480
22
0
0
Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis..
Kazama
2011
 
 
IGF-1
Animal
Cow
Amp
2
208
0
0
0
EG, SG1
RG
0
100
0
SNP exploring in the middle and terminal regions of the IGF-1 gene and..
Abdolmohammadi
2014
 
 
IGS1
Fungi
Candida..
Amp
2
33
1
0
0
EG
CFX96
0
100
33
Ribosomic DNA intergenic spacer 1 region is useful when identifying..
Gago
2014
 
 
ITS, RAPD
Plant
V. officinalis
Amp Typing
2
23
46
0
0
?
BEBO
13
0
0
Identification of Verbena officinalis based on ITS sequence analysis and..
Ruzicka
2009
 
 
ITS, SLX1, HR,..
Parasite
B. malayi
Scanning
2
5
5
280
1641
S9
RG
5
100
5
High resolution melting real-time PCR for rapid discrimination between..
Areekit
2009
 
 
ITS-2
Parasite
E. acervulina,..
Amp Typing
?
46
3
340
560
S9
RG
7
100
21
A polymerase chain reaction-coupled high-resolution melting curve..
Kirkpatrick
2009
 
 
ITS-2
Parasite
Necator..
Amp
2
58
1
0
0
S9
ABI 7500
5
100
58
Rapid Detection and Identification of Human Hookworm Infections through..
Ngui
2012
 
 
ITS1
Parasite
Leishmania..
Amp
2
1
2
0
0
0
0
0
Mucocutaneous Leishmania tropica infection in a dog from a human cutaneous..
Baneth
2014
 
 
ITS1 rDNA
Parasite
Leishmania
Amp Typing
2
300
1
265
288
S9
RG
4
100
300
Detection and identification of old world Leishmania by high resolution..
Talmi-Frank
2010
 
 
ITS1 rRNA
Parasite
Leishmania
Amp Typing
2
300
1
265
288
S9
RG
5
100
300
Detection and identification of old world Leishmania by high resolution..
Talmi-Frank
2010
 
 
ITS1, 5.8S rDNA
Fungi
Aspergillus
Mult Det
2
4
4
83
104
LCG+
HR-1
4
0
0
Multiplex single-color PCR with amplicon melting analysis for..
Erali
2006
 
 
ITS1-ITS2 rDNA
Fungi
Candida
Amp Typing
2
32
1
550
900
RL
LC480
8
0
0
High-throughput identification and quantification of candida species using..
Mandviwala
2010
 
 
ITS2
Fungi
Various Candida
Amp
1,2
46
46
200
500
EG
CFX96
16
0
0
Detection of Invasive Fungal Pathogens by Real-time PCR and..
Somogyvari
2012
 
 
ITS2
Plant
Sideritis
Detection
2
22
1
0
0
S9
RG
0
100
22
DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for..
Kalivas
2014
 
 
ITS2 rDNA
Parasite
Crypto
Amp Typing
1, 2
143
1
440
450
S9
RG
3
100
11
High resolution melting-curve (HRM) analysis for the diagnosis of..
Pangasa
2008
 
 
ITS2 rDNA
Insect
An funestus
Mult Det
2
96
5
146
587
SG1
RG
5
93.6
94
Development of multiplex real-time PCR assays for identification of..
Vezenegho
2009
 
 
ITS2 rDNA
Fungi
Candida
Amp Typing
2
22
1
141
302
RL
LC480
8
95
22
High-throughput identification and quantification of candida species using..
Mandviwala
2010
 
 
JAK1 and STAT3
Animal
Rabbit
Amp
2
253
3
120
172
EG
CFX96
0
100
759
Investigation of JAK1 and STAT3 polymorphisms and their gene–gene..
Fu
2014
 
 
kdr
Insect
An gambiae
Amp Typing
2
96
1
71
71
1.5 uM S9
RG
5
91.3
80
Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a..
Bass
2007
 
 
ldh-A, mlc-2,..
Fish
Swordfish,..
Amp, UL
2
121
3
0
0
LCG+
0
0
0
High-resolution melting analysis (HRMA): a highly sensitive inexpensive..
Smith
2010
 
 
ldlr, nsd2
Fish
Zebrafish
Detection
2
0
0
0
0
SG1
CFX96
0
0
0
A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and..
Yu
2014
 
 
LF, TLR4
Animal
Cow
Amp
2
167
3
103
137
EG
RG
4
0
0
Single nucleotide polymorphisms in immunity-related genes and their..
Carvajal
2013
 
 
Lr14a
Plant
Durum wheat..
Detection
2
176
6
56
59
S9
ABI 7500
0
0
0
Development of COS-SNP and HRM markers for high-throughput and reliable..
Terracciano
2013
 
 
Ly49 and class..
Animal
Mice
Detection
2
233
7
63
113
EG
CFX96
2
100
1631
Rapid discrimination of MHC class I and killer cell lectin-like receptor..
Lundgren
2012
 
 
MACPPE24
Bacteria
Amp
2
10
0
0
0
0
0
0
PCR amplification and high-resolution melting curve analysis as a rapid..
Castellanos
2010
 
 
matK
Plant
H. niger
Amp Typing
7
3
116
333
EG
RG
8
100
7
Quantitative high-resolution melting analysis for detecting adulterations
Mader
2011
 
 
matK
Plant
H. niger
Amp Typing
98
9
0
0
BEBO
RG
12
100
98
DNA-based identification of Helleborus niger by high-resolution melting..
Schmiderer
2010
 
 
mdr1
Parasite
Plasmodium..
Amp Typing
2
58
1
173
173
EG
RG
2
78
58
Rapid detection of point mutations in Plasmodium falciparum genes..
Andriantsoaniri..
2009
 
 
MEKK1, MEKK2,..
Plant
A. thaliana
Amp Typing
294
10
99
124
S13
CFX96
12
0
0
iTILLING: A Personalized Approach to the Identification of Induced..
Bush
2010
 
 
MP65
Fungi
Nomen..
Amp
5
3
476
476
LCG+
LC2.0
5
100
15
Rapid, simple, and low-cost identification of Candida species using..
Arancia
2011
 
 
Mstn
Fish
Takifugu..
Nested PCR
2
384
3
268
586
S9
LC480
10
100
1152
New approach for fish breeding by chemical mutagenesis: establishment of..
Kuroyanagi
2013
 
 
MSTN, GDF8
Animal
Cattle
Detection
2
1140
0
0
0
S9
RG
15
100
0
Real-time PCR genotyping and frequency of the myostatin F94L mutation in..
Vankan
2010
 
 
mt SSU rRNA
Plant
D. pinea, D...
Amp Typing
30
1
89
89
EG
RG
5
100
30
High-resolution melting analysis: a new molecular approach for the early..
Luchi
2011
 
 
mtDNA
Animal
B. primigenius
Amp Typing
75
3
74
114
LCG+
HR-1 and LS
5
100
74
Application of high-resolution melting for genotyping bovine mitochondiral..
Zhou
2011
 
 
ND4
Fish
Sharks -..
Amp
2
160
3
0
0
EG
RG
0
100
480
A mitochondrial species identification assay for Australian blacktip..
Morgan
2011
 
 
ND6 and ND4
Animal
Mice
Amp
2
22
19
109
218
LCG+
LS
19
0
0
The identification of mitochondrial DNA variants in glioblastoma multiforme
Yeung
2014
 
 
Net2
Plant
Aegilops..
Detection
2
120
12
93
145
RL
LC480
4
0
0
Application of real-time PCR-based SNP detection for mapping of Net2, a..
Matsuda
2012
 
 
OBHRM
Rodent
Lepob
Amp
2
0
1
61
61
RL
LC480
0
0
0
Efficient Method of Genotyping Ob/Ob Mice Using High Resolution Melting..
Sutter
2013
 
 
Ocp
Animal
Ochotona..
Amp
2
42
85
143
224
EG
CFX96
1
0
0
Novel genomic resources for a climate change sensitive mammal:..
Lemay
2013
 
 
oxyR
Animal
Cows, goats
Amp
2
208
2
60
79
S9
RG
2
100
416
First-Time Detection of Mycobacterium bovis in Livestock Tissues and Milk..
Ereqat
2013
 
 
p53
Fish
Zebrafish
Amp Typing
2
48
1
39
39
LCG+
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
p53
Fish
zebrafish and..
Amp
2
0
4
0
0
LS
0
0
0
High-resolution melting curve analysis for rapid detection of mutations in..
Ishikawa
2010
 
 
p53
Fish
Oryzias latipes
Detection
2
400
0
0
0
LS, LC
0
100
0
p53-Dependent suppression of genome instability in germ cells
Otozai
2014
 
 
PAL, P5CS and..
Plant
Rosa
Detection
2
3
3
204
227
RL
LC480
0
0
0
Anchoring Linkage Groups of the Rosa Genetic Map to Physical Chromosomes..
Kirov
2014
 
 
PepMV
Virus
Pepino mosaic..
Amp
2
12
2
88
128
RL
LC480
2
0
0
A new method for detection and discrimination of Pepino mosaic virus..
Hasiów-Jaroszew..
2013
 
 
pfcrt
Parasite
Plasmodium..
Amp
2
53
2
0
0
LCG+
LS
0
96.2
51
Rapid Identification of chloroquine and atovaquone drug resistance in..
Gan
2010
 
 
pfcrt, pfdhfr,..
Parasite
Plasmodium..
UL
1
66
23
0
0
LCG+
LS96
6
100
1518
Rapid, Field-Deployable Method for Genotyping and Discovery of..
Daniels
2012
 
 
Pfdhfr
Parasite
P. falciparum
UL Typing
4
4
641
641
LCG+
LC480
4
100
4
High-Throughput Genotyping of Single Nucleotide Polymorphisms in the..
Cruz
2010
 
 
phyA
Plant
Olea europaea
Amp
2
22
4
307
307
LCG+
HR-1
6
0
0
Mutation scanning and genotyping by high-resolution DNA melting analysis..
Muleo
2009
 
 
PLIN2
Animal
S. scrofa
Amp Typing
32
7
244
398
SG1
LC480
6
100
32
New SNP of the porcine perilipin 2 (PLIN2) gene, association with carcass..
Davoli
2011
 
 
pmr
Plant
Sweet Cherry..
Amp
2
114
40
0
0
RL
LC480
2
0
0
Genetic diversity and relatedness of sweet cherry (prunus avium L.)..
Fernandez
2012
 
 
pol
Animal
Pig
Nested
2
111
4
101
242
RL
LC480
0
0
0
Multiplex high resolution melting assay for estimation of Porcine..
Wynyard
2011
 
 
pol
Animal
Pig
2
14
4
101
242
RL
LC480
0
0
0
Microbiological safety of the first clinical pig islet xenotransplantation..
Wynyard
2014
 
 
PpAG, PpTFL1
Plant
P. persica
Scanning
36
6
131
149
RL
LC480
12
100
36
Mutation scanning of peach floral genes
Chen
2011
 
 
PPIB
Animal
American..
Detection
2
690
1
81
81
S9
ABI 7500
4
100
690
Allele frequency of hereditary equine regional dermal asthenia in American..
Badial
2013
 
 
rap-1c
Parasite
Babesia..
Amp
2
5
0
0
0
EG
RG
0
0
0
Typification of virulent and low virulence Babesia bigemina clones by 18S..
Thompson
2014
 
 
RPO30
Virus
Capripoxvirus
Amp
2
63
1
96
96
EG
CFX96
3
100
63
Development of a Cost-Effective Method for Capripoxvirus Genotyping Using..
Gelaye
2013
 
 
rpoB
Bacteria
Geobacillus
Amp
2
46
7
0
0
SG1
RG
0
0
0
Genotyping of Present-Day and Historical Geobacillus Species Isolates from..
Seale
2012
 
 
rpoB
Animal, Parasite
Harbor seal,..
Detection
2
48
4
0
0
S9
RG
0
0
0
Identification of a Bartonella species in the harbor seal (Phoca vitulina)..
Morick D
2009
 
 
rpoB, gltA,..
Parasite
eight..
Amp Typing
2
112
3
191
360
S9
RG
10
100
336
Detection of Bartonella spp. in wild rodents in Israel using HRM real-time..
Morick
2009
 
 
rps16
Plant
Arenaria..
Amp
2
380
2
360
390
SG1
LC480
2
99
379
Rapid identification of chloroplast haplotypes using High Resolution..
Dang
2012
 
 
rRNA
Fungus
Fusarium..
UL
2
7
1
0
0
S9
RG
7
100
7
High-resolution melting analysis allowed fast and accurate closed-tube..
Ganopoulos
2012
 
 
Ruh.7H
Plant
Barley
Detection
2
1
13
90
340
S9
RG
0
0
0
The use of high resolution melting (HRM) to map single nucleotide..
Lehmensiek
2008
 
 
SBEIIa-A
Plant
Amp Typing
56
12
152
313
LCG+
LS
6
0
0
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
SBEIIa-B
Plant
Amp Typing
14
6
19
204
LCG+
LS
6
100
14
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
SBEIIa-D
Plant
Amp Typing
53
9
151
301
LCG+
LS
6
0
0
High Resolution Melting analysis for the detection of EMS induced..
Botticella
2011
 
 
sdY
Fish
Salmo salar..
Amp
2
34
2
0
0
EG
CFX96
0
0
0
A rapid qPCR method for genetic sex identification of Salmo salar and..
d’Auriac
2014
 
 
see note
Plant
P. dulcis
Amp Typing
93
12
68
247
S9
RG
12
100
93
Mapping SNP-anchored genes using high-resolution melting analysis in almond
Wu
2009
 
 
shsp
Insect
Drosophila..
Amp
2
0
1
100
100
RL
LC480
0
0
0
Characterization of the shsp genes in Drosophila buzzatii and association..
Frydenberg
2010
 
 
SL-IR,..
Parasite
Trypanosoma..
Amp
2
38
2
0
0
S9
ABI 7500
38
50
76
Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) through..
Higuera
2013
 
 
SNP markers
Fish
O. keta
Amp Typing
190
10
118
412
BigDye
AB
3
100
95
Transcriptome sequencing and high-resolution melt analysis advance single..
Seeb
2011
 
 
SNP markers
Plant
A. sativa
Amp Typing
6
8
4
60
120
EG
CFX96
4
0
0
Model SNP development for complex genomes based on hexaploid oat using..
Oliver
2011
 
 
SNP markers
Plant
Apple
Amp Typing
2, 3, 4
6
167
0
300
LC480
0
0
0
Development of a set of SNP markers present in expressed genes of the apple
Chagne
2008
 
 
SNP markers
Plant
M. domesctica
Amp Typing
14
1
300
550
LC480
6
100
14
Development of a set of SNP markers present in expressed genes of the apple
Chagne
2008
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
167
167
S9
RG
0
97.1
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
210
210
S9
RG
0
93.6
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSII
Plant
Bread Wheat
Scanning
6
192
1
235
235
S9
RG
0
92.1
140
Simultaneous mutation detection of three homoeologous genes in wheat by..
Dong
2009
 
 
SSR markers
Plant
Grape
Amp Typing
2, 4
14
9
129
315
RL
LC480
6
0
0
A new approach to varietal identification in plants by microsatellite high..
Mackay
2008
 
 
SSR markers
Plant
Olive
Amp Typing
2, 3, 4
2
3
0
0
RL
LC480
2
0
0
A new approach to varietal identification in plants by microsatellite high..
Mackay
2008
 
 
SSR214
Plant
Oregano
Amp Typing
2
82
1
92
92
BEBO
RG
5
100
8
A strategy to setup codominant microsatellite analysis for..
Mader
2008
 
 
SSR244
Plant
Oregano
Amp Typing
2
82
1
148
148
BEBO
RG
10
100
12
A strategy to setup codominant microsatellite analysis for..
Mader
2008
 
 
SSU rDNA
Parasite
Di fragilis
Amp Typing
2
30
1
662
662
SG1
LC480
4
0
0
Genetic diversity of Dientamoeba fragilis of irritable bowel syndrome..
Hussein
2009
 
 
ssu-rRNA, bg,..
Parasite
Giardia..
Amp
2
40
2
0
0
SG1
LC480
0
0
0
Genotypes of Giardia intestinalis clinical isolates of gastrointestinal..
Al-Mohammed
2011
 
 
STS markers
Plant
White lupin
Amp Typing
2, 4, 8
2
77
91
391
S9
RG
0
0
0
High resolution melt analysis to identify and map sequence-tagged site..
Croxford
2008
 
 
t610, t208
Bacteria
Daphnia magna
Amp
2
15
2
100
200
EG
LC480
15
100
30
High-resolution melting analysis: a genotyping tool for population studies..
Kuster
2012
 
 
TBC1D1
Animal
S. scrofa
Amp Typing
778
3
32
0
EG
RG
5
100
9
The porcine TBC1D1 gene: mapping, SNP identification, and association..
Fontanesi
2011
 
 
tpi
Parasite
Giardia..
Amp
2
11
1
334
334
EG
RG
2
100
11
Application of HRM assays with EvaGreen dye for genotyping Giardia..
Zhang
2012
 
 
Tpi
Insect
Ostrinia..
UL, Amp
2
30
3
95
142
LCG+
HR-1
2
100
90
Application of High-Resolution DNA Melting for Genotyping in Lepidopteran..
Li
2012
 
 
trnL
Plant
Pinus spp.
Amp
2
74
3
0
0
S9
RG
9
100
222
Taxonomic Identification of Mediterranean Pines and Their Hybrids Based on..
Ganopoulos
2013
 
 
trnL and rpoC1
Plant
Various oils
Amp
2
18
2
0
0
6
0
0
Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel..
Ganopoulos
2013
 
 
trnL-trnF
Plant
H. niger
Amp Typing
98
6
0
0
BEBO
RG
15
100
98
DNA-based identification of Helleborus niger by high-resolution melting..
Schmiderer
2010
 
 
Tvr1
Plant
Lettuce
Scanning
2
192
5
157
648
LCG+
LS
0
0
0
Association mapping and marker-assisted selection of the lettuce dieback..
Simko
2009
 
 
UBE2
Animal
Concholepas..
Amp
2
18
1
0
0
SG1
Eco
1
0
0
Ubiquitin-conjugating enzyme E2-like gene associated to pathogen response..
Núñez-Acuña
2012
 
 
UDA
Plant
Urtica dioica
Detection
2
12
2
297
463
EG
RG
11
0
0
RAPD-PCR and real-time PCR HRM BASED GENETIC VARIATION EVALUATIONS of..
Uzonur
2013
 
 
vip3
Bacteria
Bacillus..
Amp
2
11
1
0
0
LCG+
LS
0
0
0
Detection and Identification of Vegetative Insecticidal Proteins vip3..
Li
2012
 
 
Vssc
Insect
S. scabiei
Amp Typing
2
249
7
144
144
S9
RG
4
100
20
High-resolution melt analysis for the detection of a mutation associated..
Pasay
2008
 
 
VvDXS
Plant
Vitis vinifera..
Scanning
2
242
2
125
147
LCG+
LC480
3
0
484
Development of user-friendly functional molecular markers for VvDXS gene..
Emanuelli
2013
 
 
VvNCED1
Plant
Grapevine
Detection
2
24
9
125
155
RL
LC480
2
100
216
EMS mutagenesis and qPCR-HRM prescreening for point mutations in an..
Acanda
2013
 
 
Waxy
Plant
C. annuum, C...
Amp Typing
31
8
110
457
S9
RG
6
100
31
Identification of Capsicum species using SNP markers based on high..
Jeong
2010
 
 
wdr43
Fish
Zebrafish
Amp Typing
2
48
1
50
50
LCG+
LS
3
100
24
Rapid & Efficient methods of Genotyping Zebrafish Mutants
Parant
2009
 
 
XTH
Plant
G. hirsutum
Amp Typing
2
6
1
195
195
S9
RG
2
100
6
Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from..
Michailidis
2009